Liposome Production by Dehydration-Rehydration Method

Seattle Genova has spent years developing liposome technologies, and our extensive experience qualifies us as experts in liposome preparation and manufacturing. Our liposome platform's goal is to provide you with high-quality liposome services ranging from custom liposome production, analysis, and characterization to application.

A novel method of liposome preparation has been formulated which is simple to use, utilizes mild conditions and is capable of efficient entrapment of a broad range of materials. The method is based on induction of the fusion of preformed vesicles by means of dehydration and controlled rehydration. Preliminary evidence indicates that the liposomes are mainly oligo and multilamellar. Scale-up of the procedure for industrial use is expected to be straightforward.

The method of dehydration and rehydration is also employed to prepare liposomes. By using the sonication approach, the tiny unilamellar vesicles made of phosphatidylcholine, 1,2-dioleoyl-3-(trimethylammonium) propane, cholesterol, and plasmid DNA are created. The final mixture is frozen and allowed to freeze-dry for the entire day. A controlled rehydration of the dry powders causes the creation of multilamellar dehydration-rehydration vesicles with DNA in their structure due to the binding of the cationic charges of the inner bilayers.

Advantages and Applications of Dehydration-Rehydration Method

• In all cases, the higher encapsulation efficiencies were brought by dehydration-rehydration vesicles (DRVs) and that the ratio material/lipid in sized DRVs (S-DRVs) improved three- to four-fold with respect to sized liposomes (S-MLV) received by extrusion of MLVs.

• Dehydration-rehydration vesicle methodology also promotes a novel strategy to antibody binding to liposomes.

• This technique has possible application in large-scale liposome production, since it relies only on a controlled drying and rehydration process, and does not require extensive usage of organic solvents, detergents, or dialysis systems.

• Dehydration-rehydration vesicles is a simple method that utilized in increased yield drug entrapment in liposomes.

Features of our Liposomes Custom Services

• Non-toxicity, biocompatible, and completely biodegradable

• Boosting drug efficacy

• Site avoidance effecta

• Increasing stability

• Decreasing the toxicity of drugs

Service Highlights

• Varied types of liposome-based drug delivery systems

• Controlled/sustained release drug delivery system

• Pre-formulation, formulation feasibility and prototype development

• A combination of mature preparation technologies are available

• Different identification and standardization methods

• Procedure optimization (experimental design) and aseptic filtration

References

1.       Ryman, B.E and Tyrrell, D.A 1980. Liposomes: bags of potential. Essays Biochem. 16: 49–98.

2.       Gregoriadis, G (ed.) 1984. Liposome Technology, Vols. I, II, III, CRC Press, Inc., Boca Raton, FL.

3.       Szoka, F. and Papahadjopoulos, D. 1978. A new procedure for preparation of liposomes with large internal aqueous space and high capture volume by reverse phase evaporation. Proc. Natl. Acad. Sci. USA, 75: 4194–4198.

4.       Battelle Memorial Institute 1979. Process for the preparation of liposomes in aqueous solution. British Patent Appl. No. 2001929A.